Zebrafish embryo imaging device enables positioning and long-term imaging (up to 3 days) of ~24 hpf zebrafish embryos. Embryos are placed on a side and gently pushed, tail-first into, a shallow pocket with a flexible soft roof, immobilizing embryos in consistent orientations and facilitating high- resolution longitudinal imaging of embryos in sagittal (lateral) plane. The immobilization can be further enhanced by filling the imaging chamber with a loose agarose gel.

For more information read an original article describing the chamber: "A Four-Well Dish for High-Resolution Longitudinal Imaging of the Tail and Posterior Trunk of Larval Zebrafish"